abstract：:Blood component storage allows the donor and recipient to be separated in time and space. This separation converts transfusion from a desperate clinical act into a planned, orderly healthcare logistic activity with concomitant increases in both blood product availability and safety. However, storage has the potential ...

journal_title：Journal of proteomics

authors： Hess JR

更新日期：2010-01-03 00:00:00

abstract：:Membrane proteomics offers unprecedented possibilities to compare protein expression in health and disease leading potentially to the identification of markers, of targets for therapeutics and to a better understanding of disease mechanisms. From transfusion medicine to infectious diseases, from cardiovascular affecti...

journal_title：Journal of proteomics

authors： Pasini EM,Lutz HU,Mann M,Thomas AW

更新日期：2010-01-03 00:00:00

abstract：:In recent years, affinity-based technologies have become important tools for serum profiling to uncover protein expression patterns linked to disease state or therapeutic effects. In this study, we describe a path towards the production of an antibody microarray to allow protein profiling of biotinylated human serum s...

journal_title：Journal of proteomics

authors： Rimini R,Schwenk JM,Sundberg M,Sjöberg R,Klevebring D,Gry M,Uhlén M,Nilsson P

更新日期：2009-12-01 00:00:00

abstract：:During the last two decades nitric oxide (NO) has emerged as a new chemical messenger in plant biology, which is involved in many different physiological processes, such as plant defense, transpiration and gas exchange, seed germination, and root development. Protein S-nitrosylation, the post-translational modificatio...

journal_title：Journal of proteomics

authors： Lindermayr C,Durner J

更新日期：2009-11-02 00:00:00

abstract：:The pre-treatment of biological extracts with the aim of detecting very low-abundance proteins generates complexity requiring a proper fractionation. Therefore the success of identifying all newly detectable species depends on the selected fractionation methods. In this context and starting from a human serum, where t...

journal_title：Journal of proteomics

authors： Restuccia U,Boschetti E,Fasoli E,Fortis F,Guerrier L,Bachi A,Kravchuk AV,Righetti PG

更新日期：2009-08-20 00:00:00

abstract：:Multiple reaction monitoring (MRM), commonly employed for the mass spectrometric detection of small molecules, is rapidly gaining ground in proteomics. Its high sensitivity and specificity makes this targeted approach particularly useful when sample throughput or proteome coverage limits global studies. Existing tools...

journal_title：Journal of proteomics

authors： Walsh GM,Lin S,Evans DM,Khosrovi-Eghbal A,Beavis RC,Kast J

更新日期：2009-07-21 00:00:00

abstract：:In analysing the red blood cell cytoplasmic proteome, in search for low abundance proteins, 15 amino acid (AA; Arg, Asn, Asp, Gln, Gly, His, Ile, Lys, Phe, Pro, Ser, Thr, Trp, Tyr, and Val) probes, used individually, captured a total of 787 unique gene products. Of those, 76 were found to be the common catch of all AA...

journal_title：Journal of proteomics

authors： Masseroli M,Bachi A,Boschetti E,Righetti PG

更新日期：2009-07-21 00:00:00

abstract：:We defined the transcriptomic and proteomic profiles of rat ageing skeletal muscle using a combined cDNA array, 2D- and Blue native-PAGE approach. This was allowed to obtain an overview of the interrelated events leading to the transcriptome/proteome/mitoproteome changes likely to underlie the structural/metabolic fea...

journal_title：Journal of proteomics

authors： Lombardi A,Silvestri E,Cioffi F,Senese R,Lanni A,Goglia F,de Lange P,Moreno M

更新日期：2009-05-02 00:00:00

abstract：:Due to innovative advancements in quantitative MS technologies, proteomics has evolved from taking mere "snapshots" of distinct proteomes in a defined state to monitoring, for instance, changes in abundance, location and/or posttranslational modification(s) of proteins under various conditions, thereby facilitating th...

journal_title：Journal of proteomics

authors： Oeljeklaus S,Meyer HE,Warscheid B

更新日期：2009-04-13 00:00:00

abstract：:This review is the continuation of three previously published articles [Jorrin JV, Maldonado AM, Castillejo MA. Plant proteome analysis: a 2006 update. Proteomics 2007; 7: 2947-2962; Rossignol M, Peltier JB, Mock HP, Matros A, Maldonado AM, Jorrin JV. Plant proteome analysis: a 2004-2006 update. Proteomics 2006; 6: 55...

journal_title：Journal of proteomics

authors： Jorrín-Novo JV,Maldonado AM,Echevarría-Zomeño S,Valledor L,Castillejo MA,Curto M,Valero J,Sghaier B,Donoso G,Redondo I

更新日期：2009-04-13 00:00:00

abstract：:The yeast Saccharomyces cerevisiae is able to grow under aerobic as well as anaerobic conditions. We and others previously found that transcription levels of approximately 500 genes differed more than two-fold when cells from anaerobic and aerobic conditions were compared. Here, we addressed the effect of anaerobic gr...

journal_title：Journal of proteomics

authors： Bruckmann A,Hensbergen PJ,Balog CI,Deelder AM,Brandt R,Snoek IS,Steensma HY,van Heusden GP

更新日期：2009-01-30 00:00:00

abstract：:For relative protein quantitation by mass spectrometry we metabolically labeled E. coli bacteria with (15)N-enriched diets. Proteins extracted from (15)N-labeled and unlabeled E. coli bacteria were mixed, separated by two-dimensional gel electrophoresis and enzymatically digested. The resulting tryptic peptides were a...

journal_title：Journal of proteomics

authors： Haegler K,Mueller NS,Maccarrone G,Hunyadi-Gulyas E,Webhofer C,Filiou MD,Zhang Y,Turck CW

更新日期：2009-01-30 00:00:00

abstract：:An automated image analysis system was used for protein quantification of 1862 human proteins in 47 cancer cell lines and 12 clinical cell samples using cell microarrays and immunohistochemistry. The analysis suggests that most proteins are expressed in a cell size dependent manner, and that normalization is required ...

journal_title：Journal of proteomics

authors： Lundberg E,Gry M,Oksvold P,Kononen J,Andersson-Svahn H,Pontén F,Uhlén M,Asplund A

更新日期：2008-10-07 00:00:00

abstract：:Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods have been used to provide high-resolution separation of wool intermediate filament proteins (IFPs). An improved method of extraction was developed based on a previously published method. The improved method for extraction eliminates the use of dialys...

journal_title：Journal of proteomics

authors： Paton LN,Gerrard JA,Bryson WG

更新日期：2008-10-07 00:00:00

abstract：:We carried out a comparative proteomic study to explore the molecular mechanisms that underlie the defense response of Douglas-fir (DF, Pseudotsuga menziesii) to laminated root rot, a disease caused by Phellinus sulphurascens. 2-DE was conducted on proteins extracted from roots of laboratory-grown, young DF seedlings ...

journal_title：Journal of proteomics

authors： Islam MA,Sturrock RN,Ekramoddoullah AK

更新日期：2008-10-07 00:00:00

abstract：:Hollow fiber flow field-flow fractionation (HF FlFFF) has been demonstrated as a tool for pre-fractionating proteomes by differences in molecular mass (Mr), where the resulting protein fractions are subsequently digested and analyzed by shotgun proteomics using two-dimensional liquid chromatography-electrospray ioniza...

journal_title：Journal of proteomics

authors： Kim KH,Kang D,Koo HM,Moon MH

更新日期：2008-04-30 00:00:00

abstract：:Plans for the European Proteomics Association (EuPA) were conceived and established during 2004 and 2005, and culminated in the formal inception of the organisation during the 4th HUPO World Congress held in Munich in 2005. The mission from the outset has been three-tiered and is to: i) strengthen the national Proteom...

journal_title：Journal of proteomics

authors： Dunn MJ,Gil C,Kleinhammer C,Lottspeich F,Pennington S,Sanchez JC,Albar JP,Bini L,Corrales F,Corthals GL,Fountoulakis MM,Hoogland C,James P,Jensen ON,Jiménez C,Jorrín-Novo J,Kraus HJ,Meyer H,Noukakis D,Palagi PM,Pe

更新日期：2008-04-30 00:00:00